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Friday, 02 December 2011

New insights in the life cycle of inorganic nanoparticles, SMALL (2011)

New research results that will lead to improved understandings of the impact of nanomaterials on human health and more effective strategies for detoxification of nanoparticles have recently been published in the journal Small by researchers of ICN and the University of Salzburg.

The reactivity (and potential risks) posed by inorganic nanoparticles in biological environments depends on their physical and chemical state. Biological fluids such as blood, mucus, cell culture media, and others contain a large variety of substances that can interact with and modify nanoparticles. The nanoparticle protein corona, the outcome of the absorption of proteins onto the inorganic surface, is one of the most significant alterations. This coating provides a “biological identity” to nanoparticles in those fluids and determines their interaction with cells, immune systems and other components of biological systems. As a result, detailed knowledge of the nanoparticle protein corona has emerged as a crucial aspect in understanding the biodistribution and reactivity of nanoparticles in organisms and, therefore, for the safe design of the engineered nanoparticles. As reported in Small, Dr. Eudald Casals and ICREA Prof. Victor Puntes of the ICN, along with their colleagues in the University of Salzburg, studied the surface modifications of metal (Au, Ag) and metal oxide (Fe3O4, CeO2 and CoO) nanoparticles with sizes ranging from 7 to 20 nm dispersed in commonly used cell culture medium supplemented with serum. All tested nanoparticles absorb proteins onto their surface, thereby forming a protein corona through a dynamic process evolving towards an irreversible coating (hard protein corona). Despite the fact that the studied nanomaterials have similar characteristics of hydrophobicity and surface charge, different temporal patterns of the protein corona formation were observed that can be considered a fingerprint for nanoparticle identification. Moreover, the researchers found that the protein corona formation on CoO nanoparticles reduces their adverse effects on cellular oxidative stress.

Researchers of the Inorganic Nanoparticles Group at the Catalan Institute of Nanotechnology (ICN), along with collaborators in the Department of Molecular Biology at the University of Salzburg have demonstrated the hardening (increased stability) of the corona of proteins formed around nanoparticles dispersed in biological fluids such as cell culture medium and blood serum.

Hardening of the Nanoparticle–Protein Corona in Metal (Au, Ag) and Oxide (Fe 3 O 4 , CoO, and CeO 2 ) Nanoparticles

Eudald Casals , Tobias Pfaller , Albert Duschl , Gertie J. Oostingh ,and Victor F. Puntes *

Small 2011, DOI: 10.1002/smll.201101511


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This paper is the continuation of the findings of the authors published in ACS Nano: